実績

Oda, Y., Takahashi, C., Harada, S., Nakamura, S., Sun, D., Kiso, K.,Urata, Y., Miyachi, H., Fujiyoshi, Y., Honigmann, A., et al. (2021).Discovery of anti-inflammatory physiological peptides that promote　tissue repair by reinforcing epithelial barrier formation. Sci Adv 7,eabj6895. 10.1126/sciadv.abj6895.

Ichijo, R., Kabata, M., Kidoya, H., Muramatsu, F., Ishibashi, R., Abe, K., Tsutsui, K., Kubo, H., Iizuka, Y., Kitano, S., et al. (2021). Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs. Sci Adv 7. 10.1126/sciadv.abd2575.

Cui, G., Shimba, A., Ma, G., Takahara, K., Tani-Ichi, S., Zhu, Y., Asahi, T., Abe, A., Miyachi, H., Kitano, S., et al. (2020). IL-7R-Dependent Phosphatidylinositol 3-Kinase Competes with the STAT5 Signal to Modulate T Cell Development and Homeostasis. J. Immunol. 204, 844-857.

Imayoshi, I., Tabuchi, S., Matsumoto, M., Kitano, S., Miyachi, H., Kageyama, R., and Yamanaka, A. (2020). Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3. Sci Rep 10, 3191.

Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., and Toyoshima, F. (2020). Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci Rep 10, 14120.

Kuroki, S., Maeda, R., Yano, M., Kitano, S., Miyachi, H., Fukuda, M., Shinkai, Y., and Tachibana, M. (2020). H3K9 Demethylases JMJD1A and JMJD1B Control Prospermatogonia to Spermatogonia Transition in Mouse Germline. Stem Cell Reports 15, 424-438.

Ochi, S., Imaizumi, Y., Shimojo, H., Miyachi, H., and Kageyama, R. (2020). Oscillatory expression of Hes1 regulates cell proliferation and neuronal differentiation in the embryonic brain. Development 147.

Tani-Ichi, S., Wagatsuma, K., Hara, T., Cui, G., Abe, S., Miyachi, H., Kitano, S., and Ikuta, K. (2020). Innate-like CD27. J Immunol 204, 2671-2684.

Itoh, K., Kondoh, G., Miyachi, H., Sugai, M., Kaneko, Y., Kitano, S., Watanabe, H., Maeda, R., Imura, A., Liu, Y., et al. (2019). Dephosphorylation of protamine 2 at serine 56 is crucial for murine sperm maturation in vivo. Sci. Signal. 12, 10.1126/scisignal.aao7232.

Kobayashi, T., Piao, W., Takamura, T., Kori, H., Miyachi, H., Kitano, S., Iwamoto, Y., Yamada, M., Imayoshi, I., Shioda, S., Ballabio, A., and Kageyama, R. (2019). Enhanced lysosomal degradation maintains the quiescent state of neural stem cells. Nat. Commun. 10, 5446-019-13203-4.

Qian, N., Ichimura, A., Takei, D., Sakaguchi, R., Kitani, A., Nagaoka, R., Tomizawa, M., Miyazaki, Y., Miyachi, H., Numata, T., et al. (2019). TRPM7 channels mediate spontaneous Ca(2+) fluctuations in growth plate chondrocytes that promote bone development. Sci. Signal. 12, 10.1126/scisignal.aaw4847.

Someda, M., Kuroki, S., Miyachi, H., Tachibana, M., and Yonehara, S. (2019). Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis. Cell Death Differ.

宮地　均、北野さつき、伊藤克彦、生田宏一　CRISPR/Casシステムによる遺伝子改変マウスの作製実績　第53回日本実験動物技術者協会、松山、2019年10月25日

宮地　均、北野さつき、伊藤克彦、生田宏一　京都大学ウイルス・再生医科学研究所　附属感染症モデル研究センターにおけるマウス生殖工学支援について　日本実験動物技術者協会　関東支部　REG部会　第20回特別講演会、東京、2019年11月16日

Aoyama, N., Miyoshi, H., Miyachi, H., Sonoshita, M., Okabe, M., and Taketo, M.M. (2018). Transgenic mice that accept Luciferase- or GFP-expressing syngeneic tumor cells at high efficiencies. Genes Cells 23, 580-589.

Bending, D., Martin, P.P., Paduraru, A., Ducker, C., Marzaganov, E., Laviron, M., Kitano, S., Miyachi, H., Crompton, T., and Ono, M. (2018). A timer for analyzing temporally dynamic changes in transcription during differentiation in vivo. J. Cell Biol. 217, 2931-2950.

Kuroki, S., Nakai, Y., Maeda, R., Okashita, N., Akiyoshi, M., Yamaguchi, Y., Kitano, S., Miyachi, H., Nakato, R., Ichiyanagi, K., Shirahige, K., Kimura,H., Shinkai,Y., Tachibana,M. (2018). Combined Loss of JMJD1A and JMJD1B Reveals Critical Roles for H3K9 Demethylation in the Maintenance of Embryonic Stem Cells and Early Embryogenesis. Stem Cell. Reports 10, 1340-1354.

Mori, K., Nakamura, H., Kurooka, H., Miyachi, H., Tamada, K., Sugai, M., Takumi, T., and Yokota, Y. (2018). Id2 determines intestinal identity through repression of the foregut transcription factor, Irx5. Mol. Cell. Biol.

Shimba, A., Cui, G., Tani-Ichi, S., Ogawa, M., Abe, S., Okazaki, F., Kitano, S., Miyachi, H., Yamada, H., Hara, T., Yoshikai,Y., Nagasawa,T., Schutz,G., Ikuta,K. (2018). Glucocorticoids Drive Diurnal Oscillations in T Cell Distribution and Responses by Inducing Interleukin-7 Receptor and CXCR4. Immunity 48, 286-298.e6.

Ichijo, R., Kobayashi, H., Yoneda, S., Iizuka, Y., Kubo, H., Matsumura, S., Kitano, S., Miyachi, H., Honda, T., and Toyoshima, F. (2017). Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration. Nat. Commun. 8, 508-017-00433-7.

Kuroki, S., Okashita, N., Baba, S., Maeda, R., Miyawaki, S., Yano, M., Yamaguchi, M., Kitano, S., Miyachi, H., Itoh, A., Yoshida, M., and Tachibana, M. (2017). Rescuing the aberrant sex development of H3K9 demethylase Jmjd1a-deficient mice by modulating H3K9 methylation balance. PLoS Genet. 13, e1007034.

Fukuda, M., Inoue, M., Muramatsu, D., Miyachi, H., and Shinkai, Y. (2016). Knockout mouse production assisted by Blm knockdown. J. Reprod. Dev. 62, 121-125.

Shimojo, H., Isomura, A., Ohtsuka, T., Kori, H., Miyachi, H., and Kageyama, R. (2016). Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30, 102-116.

Abe, A., Tani-Ichi, S., Shitara, S., Cui, G., Yamada, H., Miyachi, H., Kitano, S., Hara, T., Abe, R., Yoshikai, Y., and Ikuta, K. (2015). An Enhancer of the IL-7 Receptor alpha-Chain Locus Controls IL-7 Receptor Expression and Maintenance of Peripheral T Cells. J. Immunol. 195, 3129-3138.

Inoue, M., Iwai, R., Yamanishi, E., Yamagata, K., Komabayashi-Suzuki, M., Honda, A., Komai, T., Miyachi, H., Kitano, S., Watanabe, C., Teshima, W., and Mizutani, K. (2015). Deletion of Prdm8 impairs development of upper-layer neocortical neurons. Genes Cells 20, 758-770.

Kuroki, S., Akiyoshi, M., Ideguchi, K., Kitano, S., Miyachi, H., Hirose, M., Mise, N., Abe, K., Ogura, A., and Tachibana, M. (2015). Development of a general-purpose method for cell purification using Cre/loxP-mediated recombination. Genesis 53, 387-393.

Nishijima, H., Kitano, S., Miyachi, H., Morimoto, J., Kawano, H., Hirota, F., Morita, R., Mouri, Y., Masuda, K., Imoto, I., Ikuta, K., and Matsumoto, M. (2015). Ectopic Aire Expression in the Thymic Cortex Reveals Inherent Properties of Aire as a Tolerogenic Factor within the Medulla. J. Immunol. 195, 4641-4649.

Wagatsuma, K., Tani-Ichi, S., Liang, B., Shitara, S., Ishihara, K., Abe, M., Miyachi, H., Kitano, S., Hara, T., Nanno, M., et al. (2015). STAT5 Orchestrates Local Epigenetic Changes for Chromatin Accessibility and Rearrangements by Direct Binding to the TCRgamma Locus. J. Immunol. 195, 1804-1814.

Cui, G., Hara, T., Simmons, S., Wagatsuma, K., Abe, A., Miyachi, H., Kitano, S., Ishii, M., Tani-ichi, S., and Ikuta, K. (2014). Characterization of the IL-15 niche in primary and secondary lymphoid organs in vivo. Proc. Natl. Acad. Sci. U. S. A. 111, 1915-1920.

Dai, P., Harada, Y., Miyachi, H., Tanaka, H., Kitano, S., Adachi, T., Suzuki, T., Hino, H., and Takamatsu, T. (2014). Combining TGF-beta signal inhibition and connexin43 silencing for iPSC induction from mouse cardiomyocytes. Sci. Rep. 4, 7323.

Hirai, H., Fujishita, T., Kurimoto, K., Miyachi, H., Kitano, S., Inamoto, S., Itatani, Y., Saitou, M., Maekawa, T., and Taketo, M.M. (2014). CCR1-mediated accumulation of myeloid cells in the liver microenvironment promoting mouse colon cancer metastasis. Clin. Exp. Metastasis 31, 977-989.

Sakamoto, M., Ieki, N., Miyoshi, G., Mochimaru, D., Miyachi, H., Imura, T., Yamaguchi, M., Fishell, G., Mori, K., Kageyama, R., and Imayoshi, I. (2014). Continuous postnatal neurogenesis contributes to formation of the olfactory bulb neural circuits and flexible olfactory associative learning. J. Neurosci. 34, 5788-5799.

Makino, Y., Inoue, E., Hada, M., Aoshima, K., Kitano, S., Miyachi, H., and Okada, Y. (2014). Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo. Front. Cell Dev. Biol., 23 July 2014 | doi: 10.3389/fcell.2014.00030

Imayoshi, I., Isomura, A., Harima, Y., Kawaguchi, K., Kori, H., Miyachi, H., Fujiwara, T., Ishidate, F., and Kageyama, R. (2013). Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Science 342, 1203-1208.

Kuroki, S., Akiyoshi, M., Tokura, M., Miyachi, H., Nakai, Y., Kimura, H., Shinkai, Y., and Tachibana, M. (2013) JMJD1C, a JmjC Domain-Containing Protein, Is Required for Long-Term Maintenance of Male Germ Cells in Mice. Biol.Reprod.

Kuroki, S., Matoba, S., Akiyoshi, M., Matsumura, Y., Miyachi, H., Mise, N., Abe, K., Ogura, A., Wilhelm, D., Koopman, P., Nozaki, M., Kanai, Y., Shinkai, Y., and Tachibana, M. (2013) Epigenetic regulation of mouse sex determination by the histone demethylase Jmjd1a. Science. 341, 1106-1109

Tani-Ichi, S., Shimba, A., Wagatsuma, K., Miyachi, H., Kitano, S., Imai, K., Hara, T., and Ikuta, K. (2013) Interleukin-7 receptor controls development and maturation of late stages of thymocyte subpopulations. Proc.Natl.Acad.Sci.U.S.A. 110, 612-617

Deguchi, K., Nagamatsu, G., Miyachi, H., Kato, Y., Morita, S., Kimura, H., Kitano, S., Hatada, I., Saga, Y., Tachibana, M., and Shinkai, Y. (2013) Posttranscriptional regulation of histone lysine methyltransferase GLP in embryonic male mouse germ cells. Biol.Reprod. 88, 36

Huong le, T., Kobayashi, M., Nakata, M., Shioi, G., Miyachi, H., Honjo, T., and Nagaoka, H. (2013) In vivo analysis of Aicda gene regulation: a critical balance between upstream enhancers and intronic silencers governs appropriate expression. PLoS One. 8, e61433

Hara, T., Shitara, S., Imai, K., Miyachi, H., Kitano, S., Yao, H., Tani-Ichi, S., and Ikuta, K. (2012) Identification of IL-7-Producing Cells in Primary and Secondary Lymphoid Organs Using IL-7-GFP Knock-In Mice. J.Immunol. 189, 1577-1584

Imayoshi, I., Hirano, K., Kitano, S., Miyachi, H., and Kageyama, R. (2012) In vivo evaluation of PhiC31 recombinase activity in transgenic mice. Neurosci.Res. 73, 106-114

Imayoshi, I., Hirano, K., Sakamoto, M., Miyoshi, G., Imura, T., Kitano, S., Miyachi, H., and Kageyama, R. (2012) A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination. Neurosci.Res. 73, 85-91

Imayoshi, I., Tabuchi, S., Hirano, K., Sakamoto, M., Kitano, S., Miyachi, H., Yamanaka, A., and Kageyama, R. (2012) Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0. Neurosci.Res.

Liang, B., Hara, T., Wagatsuma, K., Zhang, J., Maki, K., Miyachi, H., Kitano, S., Yabe-Nishimura, C., Tani-Ichi, S., and Ikuta, K. (2012) Role of hepatocyte-derived IL-7 in maintenance of intrahepatic NKT cells and T cells and development of B cells in fetal liver. J.Immunol. 189, 4444-4450

Niwa, Y., Shimojo, H., Isomura, A., Gonzalez, A., Miyachi, H., and Kageyama, R. (2011) Different types of oscillations in Notch and Fgf signaling regulate the spatiotemporal periodicity of somitogenesis. Genes Dev. 25, 1115-1120

Takashima, Y., Ohtsuka, T., Gonzalez, A., Miyachi, H., and Kageyama, R. (2011) Intronic delay is essential for oscillatory expression in the segmentation clock. Proc.Natl.Acad.Sci.U.S.A. 108, 3300-3305

Yamazaki, D., Tabara, Y., Kita, S., Hanada, H., Komazaki, S., Naitou, D., Mishima, A., Nishi, M., Yamamura, H., Yamamoto, S., Kakizawa, S., Miyachi, H., Yamamoto, S., Miyata, T., Kawano, Y., Kamide, K., Ogihara, T., Hata, A., Umemura, S., Soma, M., Takahashi, N., Imaizumi, Y., Miki, T., Iwamoto, T., and Takeshima, H. (2011) TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance. Cell.Metab. 14, 231-241

Takeo, T., Kondo, T., Haruguchi, Y., Fukumoto, K., Nakagawa, Y., Takeshita, Y., Nakamuta, Y., Tsuchiyama, S., Shimizu, N., Hasegawa, T., Goto, M., Miyachi, H., Anzai, M., Fujikawa, R., Nomaru, K., Kaneko, T., Itagaki, Y., and Nakagata, N. (2010) Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved sperm. J.Am.Assoc.Lab.Anim.Sci. 49, 415-419

Matsui, T., Leung, D., Miyashita, H., Maksakova, I.A., Miyachi, H., Kimura, H., Tachibana, M., Lorincz, M.C., and Shinkai, Y. (2010) Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET. Nature. 464, 927-931

Takeo, T., Kaneko, T., Haruguchi, Y., Fukumoto, K., Machida, H., Koga, M., Nakagawa, Y., Takeshita, Y., Matsuguma, T., Tsuchiyama, S., Shimizu, N., Hasegawa, T., Goto, M., Miyachi, H., Anzai, M., Nakatsukasa, E., Nomaru, K., and Nakagata, N. (2009) Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature. Cryobiology. 58, 196-202

Furushima, K., Yamamoto, A., Nagano, T., Shibata, M., Miyachi, H., Abe, T., Ohshima, N., Kiyonari, H., and Aizawa, S. (2007) Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev.Biol. 306, 480-492

Hirata, T., Nakazawa, M., Yoshihara, S., Miyachi, H., Kitamura, K., Yoshihara, Y., and Hibi, M. (2006) Zinc-finger gene Fez in the olfactory sensory neurons regulates development of the olfactory bulb non-cell-autonomously. Development. 133, 1433-1443

Kanda, H., Tateya, S., Tamori, Y., Kotani, K., Hiasa, K., Kitazawa, R., Kitazawa, S., Miyachi, H., Maeda, S., Egashira, K., and Kasuga, M. (2006) MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J.Clin.Invest. 116, 1494-1505

Cui, G., Shimba, A., Jin, J., Ogawa, T., Muramoto, Y., Miyachi, H., Abe, S., Asahi, T., Tani-Ichi, S., Dijkstra, J.M., et al. (2022). A circulating subset of iNKT cells mediates antitumor and antiviral immunity. Sci Immunol 7, eabj8760. 10.1126/sciimmunol.abj8760.

実績

凍結保存

CRISPR/Cas9

トランスジェニックマウスの作製

キメラマウスの作製

Publications